题目：DNA origami:self-assembly dynamics and mechanical properties

时间：2017年9月18日（周一）  下午3:30—5:00

地点：玉泉校区信电楼117室

报告人：马志鹏博士

专家介绍

Zhipeng Ma received his Bachelor’s and Master’s degrees from the Department of Mechanical Engineering, Tianjin University, China, in 2009 and 2012, respectively, and the Ph.D. degree from the Department of Micro Engineering, Kyoto University, Japan, in 2016. Since 2016 October, he has been a Postdoctoral Researcher in the Department of Micro Engineering, Kyoto University, Japan. His research interest includes DNA nanotechnology, Micro-Electro-Mechanical-System (MEMS), and manipulation and electrical/mechanical testing of nanomaterials based on MEMS.

报告内容

DNA origami has been widely proposed for potential applications that use it as a structural tool to assemble other nanomaterials into defined static or dynamic nanostructures. The effective use of those nanostructures including their signal exchange with external systems requires the incorporation on micro systems such as micro-electro-mechanical system (MEMS). Single DNA origami as a building block is limited in size so that the construction of large-scale DNA origami having reliable mechanical properties that can bridge the gap between nanomaterials and micro systems is required. However, the scale-up methodology that meets these challenges is still lacking. The rational design and production of DNA origami with desired structural and mechanical properties, for the integration on MEMS, are also challenging due to the poor understanding of self-assembly dynamics and mechanical properties of DNA origami. We investigated the self-assembly dynamics of DNA origami and the new scale-up methodology, which is capable of stably controlling the orientations of higher-order DNA origami. And also in order to explore the capability and limitation of DNA origami as an engineering material, we characterized the global and local mechanical properties of DNA origami nanostructures by several measurement methods, such as atomic force microscopy (AFM), frequency-modulation AFM, cryo-electron microscopy. In addition, a MEMS nano tweezers device was developed for the electrical and mechanical properties measurement as well as the manipulation of DNA origami-based structures.